Genome-Wide Screening of Potential RNase Y-Processed mRNAs in the M49 Serotype Streptococcus Pyogenes NZ131

Authors

Zhiyun Chen, Department of Microbiology and Immunology, University of Oklahoma Health Sciences Center,
Rahul Raghavan, Portland State UniversityFollow
Fengxia Qi, Department of Microbiology and Immunology, University of Oklahoma Health Sciences Center,
Justin Merritt, Oregon Health and Science University
Jens Kreth, Oregon Health and Science University

Published In

MicrobiologyOpen

Document Type

Article

Publication Date

2018

Subjects

Bacterial genetics, RNA, Genomics

Abstract

RNase Y is a major endoribonuclease in Group A streptococcus (GAS) and other Gram‐positive bacteria. Our previous study showed that RNase Y was involved in mRNA degradation and processing in GAS. We hypothesized that mRNA processing regulated the expression of important GAS virulence factors via altering their mRNA stabilities and that RNase Y mediated at least some of the mRNA‐processing events. The aims of this study were to (1) identify mRNAs that were processed by RNase Y and (2) confirm the mRNA‐processing events. The transcriptomes of Streptococcus pyogenes NZ131 wild type and its RNase Y mutant (Δrny) were examined with RNA‐seq. The data were further analyzed to define GAS operons. The mRNA stabilities of the wild type and Δrny at subgene level were determined with tiling array analysis. Operons displaying segmental stability in the wild type but not in the Δrny were predicted to be RNase Y processed. Overall 865 operons were defined and their boundaries predicted. Further analysis narrowed down 15 mRNAs potentially processed by RNase Y. A selection of four candidates including folC1 (folylpolyglutamate synthetase), prtF (fibronectin‐binding protein), speG (streptococcal exotoxin G), ropB (transcriptional regulator of speB), and ypaA (riboflavin transporter) mRNAs was examined with Northern blot analysis. However, only folC1 was confirmed to be processed, but it is unlikely that RNase Y is responsible. We conclude that GAS use RNase Y to selectively process mRNA, but the overall impact is confined to selected virulence factors.

Description

This work is licensed under a Creative Commons Attribution 4.0 International License.

© 2018 The Authors. MicrobiologyOpen published by John Wiley & Sons Ltd.

Locate the Document

https://doi.org/10.1002/mbo3.671

DOI

10.1002/mbo3.671

Persistent Identifier

https://archives.pdx.edu/ds/psu/26454

Citation Details

Chen, Z., Raghavan, R., Qi, F., Merritt, J., & Kreth, J. (2018). Genome‐wide Screening of Potential RNase Y‐Processed mRNAs in the M49 Serotype Streptococcus pyogenes NZ 131. MicrobiologyOpen, e00671.