Sponsor
This material by Ecosystems and Networks Integrated with Genes and Molecular Assemblies (ENIGMA) (http://enigma.lbl.gov), a Science Focus Area Program at Lawrence Berkeley National Laboratory is based upon work supported by the US Department of Energy, Office of Science, Office of Biological, and Environmental Research under contract number DE-AC02-05CH11231. Sequencing of ancestral and early generation cocultures was supported by the National Science Foundation grant no. DEB-1453205 and DEB-1257525 to KLH. Development of the sequencing analysis pipeline was funded by the National Institute of Health under grant number R01AI141953 to NSB.
Published In
The ISME Journal
Document Type
Article
Publication Date
2-21-2021
Subjects
Evolution -- Adaptive changes
Abstract
Early evolution of mutualism is characterized by big and predictable adaptive changes, including the specialization of interacting partners, such as through deleterious mutations in genes not required for metabolic cross-feeding. We sought to investigate whether these early mutations improve cooperativity by manifesting in synergistic epistasis between genomes of the mutually interacting species. Specifically, we have characterized evolutionary trajectories of syntrophic interactions of Desulfovibrio vulgaris (Dv) with Methanococcus maripaludis (Mm) by longitudinally monitoring mutations accumulated over 1000 generations of nine independently evolved communities with analysis of the genotypic structure of one community down to the single-cell level. We discovered extensive parallelism across communities despite considerable variance in their evolutionary trajectories and the perseverance within many evolution lines of a rare lineage of Dv that retained sulfate-respiration (SR+) capability, which is not required for metabolic cross-feeding. An in-depth investigation revealed that synergistic epistasis across pairings of Dv and Mm genotypes had enhanced cooperativity within SR- and SR+ assemblages, enabling their coexistence within the same community. Thus, our findings demonstrate that cooperativity of a mutualism can improve through synergistic epistasis between genomes of the interacting species, enabling the coexistence of mutualistic assemblages of generalists and their specialized variants.
Rights
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
Locate the Document
DOI
10.1038/s41396-021-00919-9
Persistent Identifier
https://archives.pdx.edu/ds/psu/34996
Citation Details
Turkarslan, S., Stopnisek, N., Thompson, A. W., Arens, C. E., Valenzuela, J. J., Wilson, J., Hunt, K. A., Hardwicke, J., de Lomana, A. L. G., Lim, S., Seah, Y. M., Fu, Y., Wu, L., Zhou, J., Hillesland, K. L., Stahl, D. A., & Baliga, N. S. (2021). Synergistic epistasis enhances the co-operativity of mutualistic interspecies interactions. The ISME Journal. https://doi.org/10.1038/s41396-021-00919-9