Published In
Immunohorizons
Document Type
Article
Publication Date
8-2023
Subjects
Cell Developement -- Biology
Abstract
The E3 ubiquitin ligase cullin 3 (Cul3) is critical for invariant NKT (iNKT) cell development, as iNKT cells lacking Cul3 accumulate in the immature developmental stages. However, the mechanisms by which Cul3 mediates iNKT cell development remain unknown. In this study, we investigated the role of Cul3 in both immature and mature thymic iNKT cells using a mouse model with a T cell–specific deletion of Cul3. We found that mature iNKT cells lacking Cul3 proliferated and died more than wild-type cells did. These cells also displayed increased glucose metabolism and autophagy. Interestingly, we found that tight regulation of iron homeostasis is critical for iNKT cell development. Without Cul3, mature iNKT cells harbored higher levels of cytosolic iron, a phenotype associated with increased cell death. Taken together, our data suggest that Cul3 promotes iNKT cell development partially through intracellular iron homeostasis. ImmunoHorizons, 2023, 7: 235–242.
Rights
Copyright (c) 2023 The Authors
This work is licensed under a Creative Commons Attribution 4.0 International License.
Locate the Document
DOI
10.4049/immunohorizons.2300002
Persistent Identifier
https://archives.pdx.edu/ds/psu/40644
Citation Details
Yarosz, E. L., Kumar, A., Singer, J. D., & Chang, C. H. (2023). Cullin 3–Mediated Regulation of Intracellular Iron Homeostasis Promotes Thymic Invariant NKT Cell Maturation. ImmunoHorizons, 7(3), 235-242.