Generation of Novel Pikromycin Antibiotic Products Through Mutasynthesis

Authors

Shuchi Gupta, Portland State University
Venkatraman Lakshmanan, University of Minnesota - Twin Cities
Beom Seok Kim, Portland State University
Robert Fecik, University of Minnesota - Twin Cities
Kevin A. Reynolds, Portland State UniversityFollow

Sponsor

This work was supported by a grant from the NIH (R01 GM0876477).

Document Type

Post-Print

Publication Date

2009

Subjects

Antibiotics -- Metabolism, Macrolide antibiotics, Polyketide synthases, Polyketides -- Synthesis, Anti-infective agents

Abstract

Mutasynthesis in pikromycin PKS: The amenability of pikromycin polyketide synthase to mutational biosynthesis has been demonstrated. A natural triketide and its analogues, activated as N-acetyl-cysteamine thioesters, were synthesized and fed to a pikAI-deleted strain; this led to the production of new antibiotics. A vinyl analogue was found to have better antibacterial activity than pikromycin.

Description

this is the author’s version of a work that was accepted for publication in ChemBioChem. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in ChemBioChem, 9: 1609–1616 and is available online at: https://doi.org/10.1002/cbic.200700635

DOI

10.1002/cbic.200700635

Persistent Identifier

http://archives.pdx.edu/ds/psu/19067

Citation Details

Gupta, Shuchi; Lakshmanan, Venkatraman; Kim, Beom Seok; Fecik, Robert; and Reynolds, Kevin A., "Generation of Novel Pikromycin Antibiotic Products Through Mutasynthesis" (2009). Chemistry Faculty Publications and Presentations. 167.
http://archives.pdx.edu/ds/psu/19067