This work was supported by a grant from the NIH (R01 GM0876477).
Antibiotics -- Metabolism, Macrolide antibiotics, Polyketide synthases, Polyketides -- Synthesis, Anti-infective agents
Mutasynthesis in pikromycin PKS: The amenability of pikromycin polyketide synthase to mutational biosynthesis has been demonstrated. A natural triketide and its analogues, activated as N-acetyl-cysteamine thioesters, were synthesized and fed to a pikAI-deleted strain; this led to the production of new antibiotics. A vinyl analogue was found to have better antibacterial activity than pikromycin.
Gupta, Shuchi; Lakshmanan, Venkatraman; Kim, Beom Seok; Fecik, Robert; and Reynolds, Kevin A., "Generation of Novel Pikromycin Antibiotic Products Through Mutasynthesis" (2009). Chemistry Faculty Publications and Presentations. 167.