Sponsor
This work was supported by grants from the NIH-NIGMS to G.V. (GM64440) and S.B. (GM56663), from the German Research Foundation to A.M. (ME3135/1-1) and by the WCU project (305-20080089) from KMEST and grants from the National Research Foundation of Korea (NRF) (R31-2009-000-10032-0 and 2010-0011750) to M.K.
Published In
Nature Structural and Molecular Biology
Document Type
Post-Print
Publication Date
10-2010
Subjects
Transcription factors, RNA polymerases, Phosphorylation, Genetic transcription -- Regulation, RNA-protein interactions
Abstract
Phosphorylation of the C-terminal domain of RNA polymerase II controls the co-transcriptional assembly of RNA processing and transcription factors. Recruitment relies on conserved CTDinteracting domains that recognize different CTD phosphoisoforms during the transcription cycle, but the molecular basis for their specificity remains unclear. We show that the CTD-interacting domains of two transcription termination factors, Rtt103 and Pcf11, achieve high affinity and specificity both by specifically recognizing the phosphorylated CTD and by cooperatively binding to neighboring CTD repeats. Single amino acid mutations at the protein-protein interface abolish cooperativity and affect recruitment at the 3′-end processing site in vivo. We suggest that this cooperativity provides a signal-response mechanism to ensure that its action is confined only to proper polyadenylation sites where Serine 2 phosphorylation density is highest.
DOI
10.1038/nsmb.1893
Persistent Identifier
http://archives.pdx.edu/ds/psu/21526
Citation Details
Lunde, B. M., Reichow, S. L., Kim, M., Suh, H., Leeper, T. C., Yang, F., ... & Varani, G. (2010). Cooperative interaction of transcription termination factors with the RNA polymerase II C-terminal domain. Nature structural & molecular biology, 17(10), 1195.
Description
This is the authors' manuscript of an article subsequently published in Nature Structural and Molecular Biology, 2010 October; 17(10): 1195-1201. May be found at https://doi.org/10.1038/nsmb.1893.
Note: At the time of writing, Steve Reichow was affiliated with the University of Washington.