Detecting Intracellular ClO- with Ratiometric Fluorescent Signal and its Application in Civo

Authors

Jin Kang, Shanxi University
Fangjun Huo, Shanxi University
Yongbin Zhang, Shanxi University
Jianbin Chao, Shanxi University
Robert M. Strongin, Portland State UniversityFollow
Caixia Yin, Shanxi UniversityFollow

Sponsor

We thank the National Natural Science Foundation of China (No. 21672131, 21775096, 21705102), Talents Support Program of Shanxi Province (2014401), Shanxi Province Foundation for Returness (2017-026), Shanxi Province Science Foundation for Youths (201701D221061) and Scientific Instrument Center of Shanxi University, Institute of Pharmacology and Toxicology Academy of Military Medical Sciences PLA, Peop. Rep. China.

Published In

Sensors and Actuators B: Chemical

Document Type

Citation

Publication Date

11-2018

Abstract

BODIPY and coumarin were assembled into a single scaffold that was cleaved upon oxidation by ClO^-, thereby enabling ratiometric fluorescent detection ClO−. The ratiometric fluorescence response was distinctive (|₅₁₀/|₆₆₀=18.3) in the presence of ClO^-. It was accompanied by an obvious solution color change from dark-blue to colorless. The probe is highly selective and sensitive towards ClO^-, with a rapidly response time. Moreover, imaging experiments in live HeLa cells showed that the probe could readily penetrate the cell membrane and was amenable to real-time imaging of ex/endogenous ClO^-. Importanty, the probe was successfully used to detect ClO^- in vivo via imaging an arthritis nude mouse model.

Description

© 2018 Elsevier B.V. All rights reserved.

Locate the Document

https://doi.org/10.1016/j.snb.2018.07.072

DOI

10.1016/j.snb.2018.07.072

Persistent Identifier

https://archives.pdx.edu/ds/psu/26986

Citation Details

Kang, J., Huo, F., Zhang, Y., Chao, J., Strongin, R., Yin, C. (2018). Detecting intracellular ClO- with ratiometric fluorescent signal and its application in vivo, Sensors and Actuators B: Chemical, Volume 273, Pages 1532-1538.