Sponsor
This research was supported by National Institutes of Health grants GM060170, GM101983 and AI071798 to E.B
Published In
Journal of Biological Chemistry
Document Type
Article
Publication Date
1-4-2013
Subjects
Extracellular matrix proteins, Cell culture, Cell membranes, Cultures (Biology), Antiviral agents, Nucleic acids
Abstract
The matrix (MA) domain of the HIV-1 PrGag protein directs PrGag proteins to assembly sites at the plasma membrane by virtue of its affinity to the phospholipid, phosphatidylinositol-4,5-bisphosphate (PI(4,5)P₂). MA also binds to RNA at a site that overlaps its PI(4,5)P₂ site, suggesting that RNA binding may protect MA from associating with inappropriate cellular membranes prior to PrGag delivery to the PM. Based on this, we have developed an assay in which small molecule competitors to MA-RNA binding can be characterized, with the assumption that such compounds might interfere with essential MA functions and help elucidate additional features of MA binding. Following this approach, we have identified four compounds, including three thiadiazolanes, that compete with RNA for MA binding. We also have identified MA residues involved in thiadiazolane binding and found that they overlap the MA PI(4,5)P₂ and RNA sites. Cell culture studies demonstrated that thiadiazolanes inhibit HIV-1 replication, but are associated with significant levels of toxicity. Nevertheless, these observations provide new insights as to MA binding, and pave the way for the development of antivirals that target the HIV- 1 matrix domain.
DOI
10.1074/jbc.M112.399865
Persistent Identifier
http://archives.pdx.edu/ds/psu/9611
Citation Details
Alfadhli, Ayna, Henry McNett, Jacob Eccles, Seyram Tsagli, Colleen Noviello, Rachel Sloan, Claudia S. López, David H. Peyton, and Eric Barklis. "Analysis of Small Molecule Ligands Targeting the HIV-1 Matrix Protein-RNA Binding Site." Journal of Biological Chemistry 288, no. 1 (2013): 666-676.
Description
Copyright © 2012, The American Society for Biochemistry and Molecular Biology. This instance is the author’s version of a work that was accepted for publication in The Journal of Biological Chemistry. Access the most updated version of this article at doi: 10.1074/jbc.M112.399865