Published In

Journal of Biological Inorganic Chemistry

Document Type

Post-Print

Publication Date

5-30-2007

Subjects

Contrast media (Diagnostic imaging), Magnetic resonance imaging, Serum albumin, Ligand binding (Biochemistry)

Abstract

Lanthanide complexes (Eu3+, Gd3+ and Yb3+) of two different 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid tetraamide derivatives containing two (2) and four (3) O-benzyl-L-serine amide substituents were synthesized and their chemical exchange saturation transfer (CEST) and relaxometric properties were examined in the presence and absence of human serum albumin (HSA). Both Eu2 and Eu3 display a significant CEST effect from a single slowly exchanging Eu3+-bound water molecule, making these PARACEST complexes potentially useful as vascular MRI agents. Yb2 also showed a detectable CEST effect from both the Yb3+-bound water protons and the exchangeable NH amide protons, making it potentially useful as a vascular pH sensor. Fluorescence displacement studies using reporter molecules indicate that both Gd2 and Gd3 displace dansylsarcosine from site II of HSA with inhibition constants of 32 and 96 μM, respectively, but neither complex significantly displaces warfarin from site I. Water proton relaxation enhancements of 135 and 171% were observed upon binding of Gd2 and Gd3 to HSA, respectively, at 298 K and pH 7.4.

Description

This is the authors' version of an article that subsequently appeared in the Journal of Biological Inorganic Chemistry, 2007 August ; 12(6): 855–865. The version of record may be found at https://dx.doi:org/10.1007/s00775-007-0240-z.

Note: At the time of writing Mark Woods was affiliated with the University of Texas at Dallas.

DOI

10.1007/s00775-007-0240-z

Persistent Identifier

https://archives.pdx.edu/ds/psu/32562

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