First Advisor

Ov Slayden

Date of Award

6-16-2021

Document Type

Thesis

Degree Name

Bachelor of Science (B.S.) in Biology and University Honors

Department

Biology

Language

English

Subjects

Polycystic ovary syndrome, Pelvic floor -- Diseases, Cell separation

DOI

10.15760/honors.1102

Abstract

Background:

Poly Cystic Ovary Syndrome and Pelvic Floor Dysfunction are reproductive diseases with comorbidities whose novel relationship deserves further investigation.

Methods:

Levator Ani Muscle/Paravaginal Attachment was dissected from female Rhesus Macaque and subjected to tissue dissociation. Cells were treated with serum level hormones E2, T, and DHT. PCR, ICC, and IHC were used, followed by statistical analysis.

Results:

RNA analysis show differences in relative expression for endpoints ER, PR, AR, Ki-67, Col1A, and Vimentin. IHC along with ICC showed expression and localization in vivo.

Conclusions:

A novel in vitro model was created from macaque Levator Ani Muscle/Paravaginal Attachment for Pelvic Floor Dysfunction in Poly Cystic Ovary Syndrome women. Treatment of cells with androgens revealed a potential therapeutic target and possible etiology of PFD in PCOS women.

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Persistent Identifier

https://archives.pdx.edu/ds/psu/35744

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