Sponsor
This work was supported by National Institutes of Health [AA013519, AA010760, UL1GM118964, RL5GM118963, TL4GM118965, IT34GM141989-01], US Department of Veterans Affairs [BX004699], Andrews Genomics Funds, and McNair Scholars Program.
First Advisor
Angela Ozburn
Date of Award
6-2024
Document Type
Thesis
Degree Name
Bachelor of Science (B.S.) in Psychology and University Honors
Department
Psychology
Language
English
Subjects
Nicotine Use Disorders (NUDs), Nicotine Intake, Cotinine Levels, inbred High Drinking in the Dark (iHDID) mice, Drinking in the Dark (DID)
DOI
10.15760/honors.1531
Abstract
Nicotine use disorders (NUDs) pose a persistent global health threat, contributing to significant morbidity and mortality. It is a complex issue arising from a combination of different factors; thus, it is crucial to approach it from different perspectives. In this study, we aimed to determine whether the inbred High Drinking in the Dark (iHDID) mice replicate lines (iHDID-1 and iHDID-2) represent a good model for nicotine use disorders (NUDs). We measured nicotine (50µg/mL) intake in a Drinking in the Dark (DID) assay (n=12/sex/line). At the end of the DID assay, we measured serum cotinine levels using a cotinine ELISA kit. We found that iHDID-2 females consumed more nicotine than male iHDID-2 (p2= 0.688, p = 0.0057). Findings demonstrate that the iHDID mice consumed nicotine to levels that are comparable to those in previous studies and reached cotinine levels consistent with tobacco use. This suggests that iHDID mice are a stable model that can be used for testing the underlying mechanisms of NUD, and to test potential treatments that could be used to reduce nicotine use or alleviate symptoms of NUD.
Rights
In Copyright. URI: http://rightsstatements.org/vocab/InC/1.0/ This Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s).
Persistent Identifier
https://archives.pdx.edu/ds/psu/42131
Recommended Citation
Palacios, Jonathan, "Assessing Nicotine Intake and Cotinine Levels in Inbred High Drinking in The Dark (iHDID) Mice: A Potential Model for Nicotine Use Disorders (NUDs)" (2024). University Honors Theses. Paper 1499.
https://doi.org/10.15760/honors.1531