First Advisor

Bradley Buckley

Date of Award

5-31-2019

Document Type

Thesis

Degree Name

Bachelor of Science (B.S.) in Biology and University Honors

Department

Biology

Subjects

Mytilus edulis -- Effect of temperature on -- Oregon -- Pacific Coast, Mytilus edulis -- Effect of salt on -- Oregon -- Pacific Coast, Cell division, Ocean temperature

DOI

10.15760/honors.747

Abstract

Marine life is highly susceptible to stress and physiological impact through climate change, as this directly alters thermal temperatures and salinity concentrations of the ocean. The coastal species, Mytilus edulis, a mussel found variously in the northern hemisphere, was selected for cellular stress response testing. Through the investigation of temperature and salinity effects on Mytilus edulis using separation group exposure and flow cytometry analysis, it was found that salinity changes had significantly impacted the cellular division process. Individuals who received the environmentally controlled salinity concentration of 32ppt had abundant cells staged in the S phase of cellular division. Mussels exposed to salinity concentrations of 16ppt had reduced cells in the S phase, with the highest peak shifted to the left, indicative of increased cells in G0/G1 phase. In comparing the groups exposed to 13˚C versus 21˚C, similar results were seen, with the higher temperature being the advantaged group. Future testing using several varying temperatures between ranges of 5˚C to 30˚C may provide more information on how ocean warming can directly impact cellular response of Mytilus edulis.

Rights

In Copyright. URI: http://rightsstatements.org/vocab/InC/1.0/ This Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s).

Persistent Identifier

https://archives.pdx.edu/ds/psu/28917

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