Support was provided by a National Institute of Biomedical Imaging and Bioengineering Academic Research Enhancement Award (Grant no. EB007077), a National Center on Minority Health and Health Disparities Exploratory Program Grant (Grant no. MD003350), creating the CSU Center for Allaying Health Disparities through Research and Education, and awards from the Collins Medical Trust
Journal of Biophysics (Hindawi Publishing Corporation: Online)
Computational biology, Tissues -- Physiology, Cells -- Mechanical properties, Biophysics -- Mathematical models
Controlled external chemomechanical stimuli have been shown to influence cellular and tissue regeneration/degeneration, especially with regards to distinct disease sequelae or health maintenance. Recently, a unique three-dimensional stress state was mathematically derived to describe the experimental stresses applied to isolated living cells suspended in an optohydrodynamic trap (optical tweezers combined with microfluidics). These formulae were previously developed in two and three dimensions from the fundamental equations describing creeping flows past a suspended sphere. The objective of the current study is to determine the full-field cellular strain response due to the applied three-dimensional stress environment through a multiphysics computational simulation. In this investigation, the multiscale cytoskeletal structures are modeled as homogeneous, isotropic, and linearly elastic. The resulting computational biophysics can be directly compared with experimental strain measurements, other modeling interpretations of cellular mechanics including the liquid drop theory, and biokinetic models of biomolecule dynamics. The described multiphysics computational framework will facilitate more realistic cytoskeletal model interpretations, whose intracellular structures can be distinctly defined, including the cellular membrane substructures, nucleus, and organelles.
Kohles, S., Liang, Y., & Saha, A. (2012). Cytoskeletal strains in modeled optohydrodynamically stressed healthy and diseased biological cells. Journal of Biophysics (Hindawi Publishing Corporation: Online)