Financial support was provided by a National Institutes of Health, National Center on Minority Health and Health Disparities, Exploratory Program award (Grant No. MD003350), the Collins Medical Trust, the Oregon Engineering & Technology Industry Council, Saturday Academy Apprentices in Science & Engineering, and the PSU Maseeh College of Engineering and Computer Science Undergraduate Research and Mentor Program.
Ultrasonic waves, Tissue engineering, Biomechanics
Non-destructive techniques characterising the mechanical properties of cells, tissues, and biomaterials provide baseline metrics for tissue engineering design. Ultrasonic wave propagation and attenuation has previously demonstrated the dynamics of extracellular matrix synthesis in chondrocyte-seeded hydrogel constructs. In this paper, we describe an ultrasonic method to analyse two of the construct elements used to engineer articular cartilage in real-time, native cartilage explants and an agarose biomaterial. Results indicated a similarity in wave propagation velocity ranges for both longitudinal (1500-1745 m/s) and transverse (350-950 m/s) waveforms. Future work will apply an acoustoelastic analysis to distinguish between the fluid and solid properties including the cell and matrix biokinetics as a validation of previous mathematical models.
Kohles, Sean S.; Mason, Shelley S.; Adams, Anya P.; Berg, Robert J.; Blank, Jessica; Gibson, Fay; Righetti, Johnathan; Washington, Lesha S.; and Saha, Asit K., "Ultrasonic Wave Propagation Assessment of Native Cartilage Explants and Hydrogel Scaffolds for Tissue Engineering" (2012). Mechanical and Materials Engineering Faculty Publications and Presentations. 83.