Sponsor
Portland State University. Department of Biology
Date of Publication
1973
Document Type
Thesis
Degree Name
Master of Science (M.S.) in Biology
Department
Biology
Language
English
Subjects
Hemlock dwarf mistletoe, Photosynthesis, Plants -- Respiration
DOI
10.15760/etd.1689
Physical Description
1 online resource (20 Pages)
Abstract
Dark respiration rates of the aerial shoots of Arceuthobium tsugense, obtained by manometric and IRGA techniques, show production of C02to range between 155-300 µl CO2 g-1h-1 with evidence of seasonal variation. Experiments with 14C02 indicate that the aerial shoots are capable of some photosynthetic CO2 fixation, with 10-15% of the available 14C incorporated by the plant tissue in one hour.The portions of the o aerial shoots which are most active in C02 fixation are the young terminal regions. Biochemical characterization of the products of photosynthesis reveals that 80-90% of the incorporated 14C is ethanol soluble. Ten percent of the ethanol fraction is lipoidal in nature, the rest is H20 soluble. Ion exchange separation of the H20 soluble portion shows that 16-25% of the 14C activity is cationic, about 25% anionic, with the balance neutral. Aspartic acid, glutamic acid, and valine are present in the cationic fraction, with additional free amino acids indicated. IRGA experiments indicate an apparent photosynthetic CO2 fixation capacity of 80-90 percent of the ethanol fraction is lipoidal in nature, the rest is H20 soluble. Ion exchange separation of the H20 soluble portion shows that 16-25% of the 14C activity is cationic, about 25% anionic, with the balance neutral. Aspartic acid, glutamic acid, and valine are present in the cationic fraction, with additional free amino acids indicated. IRGA experiments indicate an apparent photosynthetic CO2 fixation capacity of 80-90 µl CO2 g-1 h-1, or 25-30% of the amount of C02 produced by respiration. The significance of these findings is discussed with respect to nutrition of the parasite.
Rights
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Persistent Identifier
http://archives.pdx.edu/ds/psu/11161
Recommended Citation
Miller, James Roger, "Photosynthesis and Respiration of Arceuthobium Tsugense" (1973). Dissertations and Theses. Paper 1689.
https://doi.org/10.15760/etd.1689