Translational Fidelity of a Eukaryotic Glutaminyl-tRNA Synthetase with an N-terminal Domain Appendage

Author

Aaron Bethea Rogers, Portland State UniversityFollow

Sponsor

Portland State University. Department of Chemistry

First Advisor

John Perona

Date of Publication

Summer 10-2-2014

Document Type

Thesis

Degree Name

Master of Science (M.S.) in Chemistry

Department

Chemistry

Language

English

Subjects

Aminoacyl-tRNA synthetases, RNA-protein interactions

DOI

10.15760/etd.2005

Physical Description

1 online resource (vi, 74 pages)

Abstract

Several Saccharomyces cerevisiae mutant tRNA^Q2 species (glutamine isoacceptor, CUG anticodon) were synthesized and assayed for aminoacylation activity with Saccharomyces cerevisiae glutaminyl-tRNA synthetase. The derived steady state parameters were compared to similar datasets from the literature. The mutants behaved analogously to similar mutant species based on tRNA from Escherichia coli, but with slightly relaxed specificity as revealed by comparison of k_cat/K_M values relative to wild type in vitro transcribed tRNA. Additionally the eukaryotic N-terminal domain appendage, as found in Sce glutaminyl-tRNA synthetase, is considered in light of the discovery of non-canonical aminoacyl-tRNA synthetase functions, including its role in the assembly of the multiple aminoacyl-tRNA synthetase complex.

Rights

In Copyright. URI: http://rightsstatements.org/vocab/InC/1.0/ This Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s).

Comments

Supplemental Data (SD) file descriptions:

SD1 (143 KB) - FASTA sequences of Eukaryotic and Bacterial QRS sequences used for Figure S1; can be opened with text editor

SD2 (15 KB) - FASTA sequences of Eco tRNA; can be opened with text editor SD3 (51 KB) - FASTA sequences of Sce tRNA; can be opened with text editor

SD4 (99 KB) - FASTA sequences of Hsa tRNA; can be opened with text editor

SD5 (289,388 KB) - Zip file of raw kinetic data (.gel); requires ImageQuant to open

SD6 (68 KB) - Spreadsheet (.xml) legend to eukaryotic QRS sequences in SD1; requires a spreadsheet application to open

Persistent Identifier

http://archives.pdx.edu/ds/psu/12745

Recommended Citation

Rogers, Aaron Bethea, "Translational Fidelity of a Eukaryotic Glutaminyl-tRNA Synthetase with an N-terminal Domain Appendage" (2014). Dissertations and Theses. Paper 2006.
https://doi.org/10.15760/etd.2005