Sponsor
Portland State University. Department of Biology
First Advisor
Trygve P. Steen
Term of Graduation
Summer 1974
Date of Publication
7-11-1974
Document Type
Thesis
Degree Name
Master of Science (M.S.) in Biology
Department
Biology
Language
English
Subjects
Regeneration (Biology), Nerves, Ambystoma mexicanum
DOI
10.15760/etd.2407
Physical Description
1 online resource (36 pages)
Abstract
It is known that nerves are particularly critical during the early or dedifferentiative phase of limb regeneration. During this period in the innervated limb, cells just proximal to the amputation surface dedifferentiate, migrate to the limb tip, and undergo mitosis. These processes give rise to a population of undifferentiated mesenchymatous cells capable of redifferentiating into the missing components of the newly forming regenerate. The consequences of denervation stand in stark contrast to the normal events occurring in the innervated limb, because neither a blastema nor a regenerate forms.
Results from this study indicate that during the early portion of the dedifferentiative phase in regenerates less than 2 3/4, days old the nerve apparently has little or no effect on the internal stump tissues. Of considerable interest in this regard is this study's documentation of a lack of neural influence on DNA synthesis and thus the cell cycle during the early dedifferentiative phase. Subsequently, during a transition period represented by 2 3/4 to 5 1/2 days regenerates there is some evidence for a neural influence on DNA synthesis in cells of the limb stump. Finally, on days 6 through 8, DNA synthesis is clearly nerve dependent. Since DNA synthesis is a prerequisite for mitosis, the depressed synthesis in a denervated limb precludes mitotic activity during the nerve-dependent, later portion of the dedifferentiative phase. Therefore, this research supports the idea that during the late dedifferentiative phase, including mound and early cone blastemal stages, there is a neural influence on the G1 or S phases of the cell cycle. This conclusion thereby makes progress toward explaining earlier observations of depressed mitotic activity during this period.
Rights
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Persistent Identifier
https://archives.pdx.edu/ds/psu/15733
Recommended Citation
Wald, Roberta Gail, "Effects of the Nerve During the Dedifferentiative Phase of Limb Regeneration in the Mexican Axolotl, Ambystoma mexicanum" (1974). Dissertations and Theses. Paper 2410.
https://doi.org/10.15760/etd.2407
Comments
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