First Advisor

John W. Myers

Date of Publication

12-14-1977

Document Type

Thesis

Degree Name

Master of Science (M.S.) in Biology

Department

Biology

Language

English

Subjects

Azotobacter, Genetic transformation

DOI

10.15760/etd.2613

Physical Description

1 online resource; digitized manuscript

Abstract

Previous methods for genetic transformation in Azotobacter vinelandii have employed poorly defined genetic markers or crude DNA extracts. An improved transformation technique has been developed for use in Azotobacter. The technique was used to transform several strains of Azotobacter with DNA carrying a defined genetic marker. A method for isolating pure, high molecular weight, biologically active DNA from Azotobacter is also presented. Purity of the extracted DNA was determined by standard chemical assays. The molecular weight was determined by boundary sedimentation techniques to be 18.2 megadaltons. DNA was obtained from several mutant strains of Azotobacter. Biological activity of these samples was demonstrated by using them to accomplish both intra- and interstrain transformation. Thermal denaturation profiles of several DNA samples are presented, from which guanine plus cytosine content was determined. Among the Azotobacter species examined, GC content ranged from 65.1 to 67.8%. The use of the new transformation and DNA isolation methods in taxonomic and mapping studies is discussed.

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Persistent Identifier

http://archives.pdx.edu/ds/psu/16402

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