First Advisor

Raj Solanki

Term of Graduation

2011

Date of Publication

1-1-2011

Document Type

Thesis

Degree Name

Master of Science (M.S.) in Electrical and Computer Engineering

Department

Electrical and Computer Engineering

Language

English

Subjects

Biochemical markers, Biosensors, Nanomedicine, Electrochemical sensors

DOI

10.15760/etd.274

Physical Description

1 online resource (vii, 59 pages)

Abstract

Miniaturization, integration and intelligence are the developing trends for sensor, especially for biosensors. The development of microelectronics technology is a powerful engine to full this objective. It is well known that the microelectronic fabrication process in proven technology for fabrication of integrated circuits. Advances in the field of micro-electronics and micro-mechanical devices combined with medical science have led to the development of numerous analytical devices in monitoring of a wide range of analytes. The unique properties of nanoscale materials offer excellent prospects for interfacing biological recognition events with electronic signal transduction and for designing a new generation of bio-electronic devices exhibiting novel functions. Biosensor development has the potential to meet the need for rapid, sensitive, and specific detection of pathogenic bacteria from natural sources. This work focuses on development of one such electrochemical biosensor platform and discusses different aspects related to the design of biosensor and biodetection systems. A new transducer for bio sensor applications based on 3-dimensional, comb structured interdigitated electrode arrays was chosen mainly for two reasons. Firstly, this geometry allows the monitoring of both resistivity and dielectric constant of solution, thus making interdigitated electrodes more versatile tools than other kind of transducers. Second, they present short electric field penetration depths, which make them more sensitive to changes occurring close to their surface (20 - 100 nm above the surface). This fact enables the monitoring of local changes in the vicinity of interest. Binding of analyte molecules to the chemically modified transducer surface induces important changes in the conductivity between the electrodes. Interdigitated electrodes have been employed to detect the presence of Anti-Transglutaminase (δTG) antibodies, that are established biomarkers for Celiac disease which is due to gluten allergy. The biosensor was optimized for specific and sensitive detection of this biomarker. The sensor showed a sensitivity down to picomolar(pM) concentration of the biomarker. Gold nanoparticles were further used for signal enhancement so as to bring the sensor performance closer to Enzyme linked immunosorbant assay (ELISA).

Rights

In Copyright. URI: http://rightsstatements.org/vocab/InC/1.0/ This Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s).

Comments

Portland State University. Dept. of Electrical and Computer Engineering

Persistent Identifier

http://archives.pdx.edu/ds/psu/7036

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