First Advisor

Joann S. Loehr

Date of Publication

1978

Document Type

Thesis

Degree Name

Master of Science (M.S.) in Chemistry

Department

Chemistry

Language

English

Subjects

Glutamic acid, Ligands, Hemoerythrin

DOI

10.15760/etd.2778

Physical Description

1 online resource (46 p.)

Abstract

The amino acid sequence determination of Phascolopsis gouldii hemerythrin in the region of the proposed iron ligand at position 58 was the main objective of this research endeavor. Generation of a large peptide was pursued by trypsin digestion of citraconylated hemerythrin producing peptide 50-113 for sequenator analysis. Detection of the phenylthiohydantoin amino acid derivatives by gas-liquid and high-performance-liquid chromatography yielded unambiguous sequence elucidation through the region of interest identifying residue 58 as glutamic acid.

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Comments

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Persistent Identifier

http://archives.pdx.edu/ds/psu/17083

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