Sponsor
Portland State University. Department of Biology
First Advisor
Lester J. Newman
Term of Graduation
Fall 1980
Date of Publication
10-1-1980
Document Type
Thesis
Degree Name
Master of Science (M.S.) in Biology
Department
Biology
Language
English
Subjects
Heterochromatin, Gene mapping
DOI
10.15760/etd.3085
Physical Description
1 online resource (v, 57, [6] pages)
Abstract
A study was conducted to cytologically map certain subsets of constitutive heterochromatin onto specific portions of human chromosomes. This involved sequentially staining metaphase chromosomes from lymphocytes first with the Centromeric Dot, Giemsa-11, G-banding or Lateral Asymmetry staining techniques, which are cytochemical methods which stain particular chromosomes or chromosomal regions. Then those same metaphase chromosomes were stained using the C-banding technique, a method which is believed to denote constitutive heterochromatin. With the exception of the G-banding technique, areas depicted by the special staining techniques were found to reside only within the areas stained by the C-band technique and are thus believed to be subsets of constitutive heterochromatin. In addition to portions of the C-band regions, G-banding stained regions throughout the chromosome arms but, only those residing within the C-band regions were considered to be subsets of constitutive heterochromatin. It was found that those regions identified by the Giemsa-11, Centromeric Dot and G-banding techniques were mutually exclusive of one another and occupied discrete regions within the C-bands.
Rights
In Copyright. URI: http://rightsstatements.org/vocab/InC/1.0/ This Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s).
Persistent Identifier
https://archives.pdx.edu/ds/psu/18046
Recommended Citation
Donlon, Timothy Atchison, "Cytologic Characterization of Human Constitutive Heterochromatin" (1980). Dissertations and Theses. Paper 3092.
https://doi.org/10.15760/etd.3085
Comments
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