Sponsor
Portland State University. Department of Biology
First Advisor
Deborah A. Duffield
Term of Graduation
Winter 1990
Date of Publication
2-2-1990
Document Type
Thesis
Degree Name
Master of Science (M.S.) in Biology
Department
Biology
Language
English
Subjects
Peptides -- Analysis, Myoglobin -- Analysis, High performance liquid chromatography, Cetacea -- Evolution
DOI
10.15760/etd.5970
Physical Description
1 online resource (3, ix, 123 pages)
Abstract
Due to the large gaps in the fossil record, the evolutionary history of the mammalian order Cetacea is incomplete and controversial. Increasingly researchers are utilizing molecular and biochemical procedures to supplement cetacean paleontology. One of these methods is the comparison of amino acid sequences of myoglobin among species of this order. since this method is time-consuming and expensive, an alternative procedure is desirable.
As a candidate for such an alternative, peptide mapping was performed on selected species of cetaceans. This mapping was done utilizing High Performance Liquid Chromatography (HPLC). Myoglobin was extracted from skeletal muscle, purified, digested with trypsin, the digest separated on a C-18 reversed phase column, and eluted with a gradient of increasing acetonitrile concentration. The eluent was monitored with ultraviolet light at 220 nm, and the absorbance of individual peptides was integrated and plotted. The characteristic peptide maps produced for each sample were compared, and the number of differences between samples tabulated in a difference matrix. Based on the amount of peptide similarity, a phylogenetic tree was constructed for the species represented. The identity of each peptide peak was determined through the use of retention coefficients.
Rights
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Persistent Identifier
https://archives.pdx.edu/ds/psu/23466
Recommended Citation
Hayteas, David Lawrence, "HPLC Analysis of Myoglobin Tryptic Peptides From Selected Species of Cetaceans" (1990). Dissertations and Theses. Paper 4086.
https://doi.org/10.15760/etd.5970
Comments
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