Sponsor
Portland State University. Department of Biology
First Advisor
David Clark
Term of Graduation
Fall 1994
Date of Publication
11-4-1994
Document Type
Thesis
Degree Name
Master of Science (M.S.) in Biology
Department
Biology
Language
English
Subjects
Neutrophils, Trichinella, Mice -- Parasites
DOI
10.15760/etd.6675
Physical Description
1 online resource (v, 31 pages)
Abstract
The Genus Trichinella is currently thought to consist of 8 separate species. Several methods have been used to help differentiate these. In this study the ability of three of these species, T. spiralis, T. pseudospiralis, and T. sp. 3 to independently infect MRL++ mice was compared at 5, 7, 11, 18 and 20 days post infection (dpi) with the hope of discovering new distinguishing characteristics. Of interest was the development of inflammation through the accumulation of neutrophils associated with larvae of Trichinella in mouse skeletal muscle. This was evaluated with routine histological stains, endogenous peroxidase staining and immunohistochemical staining specific for neutrophil myeloperoxidase. The inflammatory response in skeletal muscle varied considerably among these three species with neutrophil accumulation being very heavy in the later stages of T. spiralis infection, mild in T. sp. 3 and virtually nonexistent in T. pseudospiralis infections. Also, the appearance of neutrophils was closely correlated with the development of a nurse cell or cyst in the larvae that develop these structures. In T. pseudospiralis where cyst formation is absent, there consistently was a lesser degree of inflammation. These results suggest a possible role of neutrophils involved in inflammation or the process of inflammation itself in the production of nurse cell formation as well as confirming previously described anti inflammatory capabilities of T. sp. 3 and T. pseudospiralis.
Rights
In Copyright. URI: http://rightsstatements.org/vocab/InC/1.0/ This Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s).
Persistent Identifier
https://archives.pdx.edu/ds/psu/27974
Recommended Citation
Prulhiere, Jon Darin, "A Comparison of Neutrophil Migration in the Mouse in Response to Infection with Three Species of Trichinella" (1994). Dissertations and Theses. Paper 4791.
https://doi.org/10.15760/etd.6675
Comments
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