Portland State University. Department of Biology.
Date of Award
Master of Science (M.S.) in Biology
1 online resource (v, 31 p.)
Neutrophils, Trichinella, Mice -- Parasites
The Genus Trichinella is currently thought to consist of 8 seperate species. Several methods have been used to help differentiate these. In this study the ability of three of these species, T. spiralis, T. pseudospiralis, and T. sp. 3 to idependantly infect MRL++ mice was compared at 5, 7, 11, 18 and 20 days post infection ( dpi) with the hope of discovering new distinguishing characteristics. Of interest was the development of inflammation through the accumulation of neutrophils associated with larvae of Trichinella in mouse skeletal muscle. This was evaluated with routine histological stains, endogenous peroxidase staining and immunohistochemical staining specific for neutrophil myeloperoxidase. The inflammatory response in skeletal muscle varied considerably among these three species with neutrophil accumulation being very heavy in the later stages of T. spiralis infection , mild in T. sp. 3 and virtually nonexistant in T. pseudospiralis infections. Also, the appearance of neutrophils was closely correlated with the development of a nurse cell or cyst in the larvae that develop these structures. In T. pseudospiralis where cyst formation is absent, there consistently was a lesser degree of inflammation. These results suggest a possible role of neutrophils involved in inflammation or the process of inflammation itself in the production of nurse cell formation as well as confirming previously described antiinflammatory capabilities of T. sp. 3 and T. pseudospiralis.
Prulhiere, Jon Darin, "A Comparison of Neutrophil Migration in the Mouse in Response to Infection with Three Species of Trichinella" (1994). Dissertations and Theses. Paper 4791.