First Advisor

Jeffrey D. Singer

Term of Graduation

Winter 2022

Date of Publication

2-22-2022

Document Type

Thesis

Degree Name

Master of Science (M.S.) in Biology

Department

Biology

Language

English

Subjects

Protein binding, Ubiquitin

DOI

10.15760/etd.7790

Physical Description

1 online resource (v, 62 pages)

Abstract

Cul3 is the major component of an E3 ligase in human cells. Cul3 was initially identified in the Singer lab as a protein that binds and degrades cyclin E, and subsequent studies have shown it to be part of a complex that is involved in a multitude of biological functions. The nature of this complex, its constituents, its regulation, and its dynamics is just beginning to be understood. The research presented here utilizes a series of two hybrid screens to identify families of interactomes with Cul3 at the center. This information will complement other work in the lab in which proteomics has been utilized. In summary, this work identifies potential binding cohorts within the E3 ligase complex and suggests future work to unravel mechanistic pathways that are regulated by Cul3.

Rights

© 2022 Elyse Reitter

In Copyright. URI: http://rightsstatements.org/vocab/InC/1.0/ This Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s).

Persistent Identifier

https://archives.pdx.edu/ds/psu/37354

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