Portland State University. Department of Biology
Jeffrey D. Singer
Term of Graduation
Date of Publication
Master of Science (M.S.) in Biology
1 online resource (v, 62 pages)
Cul3 is the major component of an E3 ligase in human cells. Cul3 was initially identified in the Singer lab as a protein that binds and degrades cyclin E, and subsequent studies have shown it to be part of a complex that is involved in a multitude of biological functions. The nature of this complex, its constituents, its regulation, and its dynamics is just beginning to be understood. The research presented here utilizes a series of two hybrid screens to identify families of interactomes with Cul3 at the center. This information will complement other work in the lab in which proteomics has been utilized. In summary, this work identifies potential binding cohorts within the E3 ligase complex and suggests future work to unravel mechanistic pathways that are regulated by Cul3.
© 2022 Elyse Reitter
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Reitter, Elyse, "A Tale of Two-Hybrids: Investigating the Interactomes of Cullin-Associated Proteins" (2022). Dissertations and Theses. Paper 5919.