First Advisor

Jonathan J. Abramson

Term of Graduation

Fall 1997

Date of Publication

12-12-1997

Document Type

Thesis

Degree Name

Master of Science (M.S.) in Physics

Department

Physics

Language

English

Subjects

Calcium channels, Temperature, Sarcoplasmic reticulum

Physical Description

1 online resource (iii, 58 pages)

Abstract

The Ca2+ release protein/ryanodine receptor from skeletal muscle sarcoplasmic reticulum is shown to be modulated in a unique manner by small changes in temperature. Thiol oxidants and reducing agents modify channel function in a biphasic manner at 40° C, while at 34° C the channel exhibits only a monophasic behavior. Elevated temperature appears to expose a second set of reactive groups that, when either oxidized or reduced, alter function. Previous studies have focused on the properties of the release mechanism at or below 37° C. This study shows that at 40° C, which more reflects the body temperature of the species used, there are important functional differences in the sensitivity of the ryanodine receptor to activation by Ca2+ and GSSG, and to inhibition induced by the addition of thiol reducing agents. These results are relevant to normal channel function and alteration in receptor behavior during oxidative stress.

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In Copyright. URI: http://rightsstatements.org/vocab/InC/1.0/

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Persistent Identifier

https://archives.pdx.edu/ds/psu/44690

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