Presentation Type

Poster

Start Date

5-8-2024 11:00 AM

End Date

5-8-2024 1:00 PM

Subjects

Evolutionary biology in plants, Plant genetics--Congresses.

Advisor

Cruzan, Mitch

Student Level

Undergraduate

Abstract

A lack of genomic studies examining gametophytic selection and selective embryo abortion—which occur during the reproduction of angiosperms—leaves questions regarding the adaptive and evolutionary effects of these processes. Analyzing deviations from Mendelian segregation offers an avenue for identifying loci targeted by GS and SEA, and their contributions to purging of genetic load. However, other selective processes such as meiotic drive and cytonuclear interactions, as well as pollen and ovule abortion, can cause distortion. To distinguish the effects of GS and SEA from other causes of distortion, we will perform reciprocal crosses between highly homozygous and highly heterozygous individuals of Mimulus guttatus and conduct pooled sequencing of pollen and seeds. Given that a large fraction of the genome is expressed in pollen and seeds, we expect a large proportion of the genome to exhibit segregation distortion due to GS and SEA.

Creative Commons License or Rights Statement

Creative Commons Attribution 4.0 License
This work is licensed under a Creative Commons Attribution 4.0 License.

Persistent Identifier

https://archives.pdx.edu/ds/psu/41902

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May 8th, 11:00 AM May 8th, 1:00 PM

Selection During Reproduction in Mimulus guttatus

A lack of genomic studies examining gametophytic selection and selective embryo abortion—which occur during the reproduction of angiosperms—leaves questions regarding the adaptive and evolutionary effects of these processes. Analyzing deviations from Mendelian segregation offers an avenue for identifying loci targeted by GS and SEA, and their contributions to purging of genetic load. However, other selective processes such as meiotic drive and cytonuclear interactions, as well as pollen and ovule abortion, can cause distortion. To distinguish the effects of GS and SEA from other causes of distortion, we will perform reciprocal crosses between highly homozygous and highly heterozygous individuals of Mimulus guttatus and conduct pooled sequencing of pollen and seeds. Given that a large fraction of the genome is expressed in pollen and seeds, we expect a large proportion of the genome to exhibit segregation distortion due to GS and SEA.