Published In
Biorxiv : the Preprint Server for Biology
Document Type
Pre-Print
Publication Date
11-24-2025
Subjects
Bacteria and archaea -- Biology
Abstract
The study of temperature limits has transformed our knowledge of the boundaries of life but has been largely focused on bacteria and archaea. We isolated a novel geothermal amoeba, , that divides at 63°C (145.4°F), establishing a new record for the upper temperature limit across all eukaryotes. We demonstrated cellular proliferation with growth experiments and visualized mitosis expansion microscopy. Using high-temperature live-cell imaging, we quantified movement up to 64°C. We assembled the genome of and using comparative genomics found an enrichment of genes related to proteostasis, genome stability, and sensing the external environment. Taken together, our findings challenge the current paradigm of temperature constraints on eukaryotic cells and reshape our understanding of where and how eukaryotic life can persist.
Rights
© Copyright the author(s) 2025
Locate the Document
https://doi.org/10.1101/2025.11.24.690213
DOI
10.1101/2025.11.24.690213
Persistent Identifier
https://archives.pdx.edu/ds/psu/44392
Publisher
Cold Spring Harbor Laboratory
Citation Details
Published as: Rappaport, H. B., Petek-Seoane, N. A., Tyml, T., Mikus, F., Labutti, K., Ani, G., Niblo, J. K., MacVicar, E., Shepherd, R. M., de la Higuera, I., Lord, S. J., Dey, G., Wolfe, G. V., Dudin, O., Sukenik, S., Katz, L. A., Stedman, K. M., Skruber, K., Schulz, F., … Oliverio, A. M. (2025). A geothermal amoeba sets a new upper temperature limit for eukaryotes.
Description
This is the author’s version of a work that was accepted for publication. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published A geothermal amoeba sets a new upper temperature limit for eukaryotes. 2025. https://doi.org/10.1101/2025.11.24.690213