Sponsor
This work was funded by the US -National Science Foundation grant DEB -2409507 to A. -L. Reysenbach. The US -National Science Foundation grants OCE -1558795, 1235432, 0937404, 0752469, 0728391, 0242038, 0118240, 9714302 provided funding for the many collections.
Published In
Scientific Data
Document Type
Post-Print
Publication Date
1-16-2026
Subjects
Metagenomes -- deep-sea hydrothermal vents, thermophiles, Archaea
Abstract
Actively venting high temperature deep-sea hydrothermal vent deposits along tectonic spreading centers and in backarc basins harbor a rich diversity of thermophilic Bacteria and Archaea, many of which have no representatives in cultivation nor any genomic representation in databases. Here, in order to produce a global-scale time series metagenomic resource for studying the microbial functional and genomic diversity in these high temperature ecosystems, we obtained 70 metagenomes from collections across spatial and temporal gradients from 21 different vent fields spanning 16 years (1993-2009). The dataset (Deep-Sea Hydrothermal Vent dataset (DSV70)) includes 3.56 Tbp of raw DNA sequence reads, that have been assembled to produce 7,422 medium- to high-quality (based on CheckM2) metagenome-assembled genomes (MAGs) of Bacteria (6,063 MAGs) and Archaea (1,359 MAGs). Collectively, this DSV70 dataset and the published 40 metagenomes from more recent deep-sea collections (2004 to 2018), represent a valuable resource for exploring the functional and phylogenomic diversity of the deep-sea hydrothermal microbiomes, and provide many reference genomes for studies in the taxonomy and systematics of poorly studied microbial lineages. Further, with the interest in mining the mineral resources at deep-sea vents, the DSV70 provides a genomic legacy for monitoring impacts on the microbial communities in these systems.
Rights
Copyright (c) 2026 The Authors
This work is licensed under a Creative Commons Attribution 4.0 International License.
DOI
10.1038/s41597-026-06612-w
Persistent Identifier
https://archives.pdx.edu/ds/psu/44442
Publisher
Springer Science and Business Media LLC
Citation Details
St. John, E., & Reysenbach, A.-L. (2026). Global deep-sea hydrothermal deposit metagenomes and metagenome-assembled genomes over time and space. Scientific Data.
Description
This is the author’s version of a work that was accepted for publication.Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published as: (2026). Global deep-sea hydrothermal deposit metagenomes and metagenome-assembled genomes over time and space. Scientific Data.