Sponsor
The research was supported by the key Research and Development Program of China (#2016YFC0402204 and 2016YFC0402207), the Major Science and Technology Project for Watershed Pollution Control and Management (#2017ZX07108), the National Natural Science Foundation of China (#3130044, 231400367 and 41601332), the Key Research Project of Colleges and Universities of Henan Province Education Department (#16A210012).
Published In
Water
Document Type
Article
Publication Date
1-2018
Subjects
Plankton, Reservoir ecology, Hydrology, Drinking water -- Analysis
Abstract
Drinking water safety is increasingly perceived as one of the top global environmental issues. Plankton has been commonly used as a bioindicator for water quality in lakes and reservoirs. Recently, DNA sequencing technology has been applied to bioassessment. In this study, we compared the effectiveness of the 16S and 18S rRNA high throughput sequencing method (HTS) and the traditional optical microscopy method (TOM) in the bioassessment of drinking water quality. Five stations reflecting different habitats and hydrological conditions in Danjiangkou Reservoir, one of the largest drinking water reservoirs in Asia, were sampled May 2016. Non-metric multi-dimensional scaling (NMDS) analysis showed that plankton assemblages varied among the stations and the spatial patterns revealed by the two methods were consistent. The correlation between TOM and HTS in a symmetric Procrustes analysis was 0.61, revealing overall good concordance between the two methods. Procrustes analysis also showed that site-specific differences between the two methods varied among the stations. Station Heijizui (H), a site heavily influenced by two tributaries, had the largest difference while station Qushou (Q), a confluence site close to the outlet dam, had the smallest difference between the two methods. Our results show that DNA sequencing has the potential to provide consistent identification of taxa, and reliable bioassessment in a long-term biomonitoring and assessment program for drinking water reservoirs.
Rights
© 2018 by the authors. Licensee MDPI, Basel, Switzerland.
This work is licensed under a Creative Commons Attribution 4.0 International License.
Locate the Document
DOI
10.3390/w10010082
Persistent Identifier
http://archives.pdx.edu/ds/psu/24347
Citation Details
Gao, W., Chen, Z., Li, Y., Pan, Y., Zhu, J., Guo, S., ... & Huang, J. (2018). Bioassessment of a Drinking Water Reservoir Using Plankton: High Throughput Sequencing vs. Traditional Morphological Method. Water, 10(1), 82.
Included in
Environmental Microbiology and Microbial Ecology Commons, Environmental Sciences Commons, Hydrology Commons